Department of Physics (Biophysics), University of Manchester

Professor Jian Ren Lu

JRL worked in Oxford for 5 years and so there are many joint papers. A major collaboration following JRL's time in Oxford was on protein adsorption and protein fouling of ceramic membranes (also with Professor Zhang Feng Cui at Engineering Science, Oxford). Neutron reflection was used to study the adsorption characteristics of globular proteins at the aqueous solution/silica interface (adsorbed amount and thickness of the layer) and the results correlated with in situ studies of microporous ceramic membranes using neutron small angle scattering. The extent to which contrast variation and neutron reflection can be used to follow the removal of protein from a surface is shown in the two figures below, which show how the amount of bovine serum albumin (BSA) and its layer thickness can be followed as the increase in the concentration of the contacting solution of the surfactant sodium dodecyl sulphate. Contrast variation can be used to follow the process of removal of the BSA from the surface in some detail. Note that the colour conventions are as already described in Neutron Reflection.

The work is more fully described in Binding of surfactants on to preadsorbed layers of bovine serum albumin at the silica-water interface, Lu, Su, Thomas, J. Phys. Chem. B, 102, 10307-10315 (1998)

Some simple results from the in situ neutron small angle scattering to study protein fouling of a membrane are shown in the diagram below for BSA and are described in more detail in Identification of the location of protein fouling on ceramic membranes under dynamic filtration conditions, Su, Lu, Cui, Thomas, Heenan, J. Membrane Sci., 163, 265-275 (1999)

Jian Lu's website is at